Volume 14, no. 2
Pages 87-89

Objective: to study how original sensitivity to hypoxia influences immunitydependent suppression of microsomal oxidation in stressed and unstressed animals. Materials and methods: we used pressure chamber to model hypoxia. Animals were divided into 3 groups: high-resistant (HR), low-resistant (LR) and medium-resistant (MR) to hypoxia. Then animals with different levels of resistance to hypoxia were exposed to immobilization stress by intraperitoneal administration of ram erythrocytes. 5 days later animals were sacrificed. We estimated content of cytochromes P450 and b5 in liver microsomes and assessed oxidoreductase activity. Findings: high-resistant animals in comparison with low-resistant animals have decreased activity of NADP cytochrome P450 reductase, meanwhile low-resistant animals have increased level of this activity. Immunization combined with previously provoked stress led to decrease in cytochrome P450 concentration in high-resistant animals and, vice versa, to increase of this concentration in low-resistant animals. Conclusion: microsomal oxidation sensitivity to immunization effect depends on original resistance to hypoxia. Resistance to hypoxia also influences modifying effect of previous stress on immunity-dependent changes of microsomal oxidation.

Full text

Sources:

1. Grek O.R., Efremov A.V, Sharapov V.I. Gipobaricheskaya gipoksiya i metabolizm ksenobiotikov [Hypo-baric Hypoxia and Metabolism of Xenobiotics]. Moscow, GEOTAR-Media Publ., 2007, 120 p.

2. Sibiryak S.V., Sibiryak S.V., Vakhitov V.A., Kurchatova N.N. Tsitokhrom R450 i immunnaya sistema [Cytochrome P450 and the Immune System]. Ufa, Guillem Publ., 2003. 176 р.

3. Volchegorskiy I.A., Dolgushin I.I., Kolesnikov O.L., Tseylikman V.E. Eksperimental'noe modelirovanie i laboratornaya otsenka adaptivnykh reaktsiy organizma [Experimental Modeling and Laboratory Evaluation of Adaptive Reactions of the Organism]. Chelyabinsk, South Ural St. Univ. Publ., 2000, 167 p.

4. Blalock J.E. The Immune System as a Sensory Organ. J. Immunol, 1984, vol. 132, no. 3, pp. 1067–1070.